Surfactant protein mRNA levels in alveolar type II cells. Real-time PCR using total RNA from freshly isolated (uncultured) AT II cells was performed for quantification of mRNA levels of SP-A, SP-B and SP-C (n = 6, each). The results of triplicate analysis for each sample were paired between the control and ASCFTR samples and expressed as percentage of control. The mRNA levels (normalized to the house keeping gene RPL13A) for all three proteins were similar in the two groups. The results were considered significant at ≥ 200% (≥ 2-fold) of the Control.
Gad et al. BMC Cell Biology 2009 10:24 doi:10.1186/1471-2121-10-24