Lung surfactant secretion in alveolar type II cells isolated from control and ASCFTR rats. Alveolar type II cells were isolated from lungs of paired Control and ASCFTR rats. The cells were cultured for 20–22 h with [methyl-3H]choline to label cell phosphatidylcholine (PC). Following this culture period, adherent cells were incubated for 2 h without (basal secretion) or with addition of 1 mM ATP or 80 nM phorbol 12-Myristate 13-Acetate (PMA). The basal secretion was 1.05 ± 0.19% and 0.82 ± 0.11%, n = 6, in the control and ASCFTR cells, respectively (P > 0.05). The ATP and PMA-stimulated secretion is expressed as percent of the basal secretion. * P < 0.05 in comparison to the Control cells.
Gad et al. BMC Cell Biology 2009 10:24 doi:10.1186/1471-2121-10-24