Effect of mimitin gene silencing on proliferation of HepG2 cells. Cells were transfected with silencing RNA specific for mimitin (50 nM), or for GAPDH (100 nM, positive control), or unspecific siRNA (negative control, 50 nM) or were treated with transfection reagent siPORT NeoFX alone (blank). At 72 h post transfection cells were incubated with BrdU (5 μM) for 3 h and incorporation was determined with Cell Proliferation ELISA. A. Cell proliferation is shown relative to the non-sepcific siRNA (control). Values were calculated from absorbance readings at 450 nm and represent the mean of three independent experiments with standard deviation (*p < 0.05, **p < 0.02 compared to control). B. Representative Western blot analysis with 20 μg protein from simultaneously seeded 12-well plates using anti-GAPDH or anti-mimitin antibodies.
Wegrzyn et al. BMC Cell Biology 2009 10:23 doi:10.1186/1471-2121-10-23