Characterization of ALP107-273 protein. A) Domain architecture of seven human PDZ_LIM proteins. B) SDS-PAGE of the proteins used in SPR measurements. R2-R3 denotes α-actinin spectrin repeats 2–3, R1-R4 spectrin repeats 1–4. ALP denotes the ALP107-273 fragment. Labelled ALP107-273 used in NMR experiments had a similar purity to the non-labelled form. C) Size exclusion chromatography showed that ALP107-273 eluted faster than expected based on its calculated monomer molecular weight (18 265 Da).
Klaavuniemi et al. BMC Cell Biology 2009 10:22 doi:10.1186/1471-2121-10-22