Reverse transcription (RT)-PCR arrays for gene expression of tight junction proteins in response to challenge of H413 epithelial monolayers with anti-CD24 antibody. (A) H413 clone-1 cells challenged with anti-CD24 peptide compared to isotype IgG1 control antibody for RNA extracted 3 h after addition of antibody. RT-PCR (30 cycles) reaction products were visualized on a 2% agarose gel after staining with ethidium bromide (representative of three separate experiments). (B) Bar graph showing mean values from three independent experiments (± s.e.m.) for anti-CD24 peptide antibody studies. The values for each experiment were normalized against the expression of β-actin (assigned as 100) and compared with the control sample and analyzed by paired t-test. * Refers to P < 0.05; four tight junction genes: ZO-1, ZO-2, occludin and par-3 showed significant differences that were confirmed by real-time quantitative RT-PCR analysis in Table 1. Expression of genes encoding claudin-3 and par-6 was not detected in control samples by real-time quantitative RT-PCR analysis.
Ye et al. BMC Cell Biology 2009 10:2 doi:10.1186/1471-2121-10-2