Table 1

HAC daily loss rate

HAC (%) 0 days off selection

HAC (%)

150 days off selection

Loss rate

(%)


17α-HPRT HAC

AG6-1 (h)

96

86

0.07

Sag1.1 (m)

80

20

0.92

Sag 1.2 (m)

85

6.6

1.68

Sag 2.2 (m)

88

6

1.77

Sag 2.3 (m)

88.6

10

1.44


17α-HAC

LJ2-1 (h)

92

86

0.04

SM1-1(C6) (m)

100

Nd

*5.20


The percentage of HAC containing metaphases in human (h) and murine derivatives (m), in absence of selection at 0 and 150 days is shown. The last column on the right indicates the percentage loss rate (%) after 150 days in the absence of selection, calculated by the formula Nn = N0 × (1-R)n, where N0 is the number of metaphase chromosome spreads showing HAC in the cells cultured under selection, Nn is the number of HAC-containing metaphase chromosome spreads after n days of culture in the absence of selection, and R is the daily rate of loss. Each table cell shows the human parentals (at the top) and their mouse derivatives. nd: none detected

* Loss rate after 30 days [8].

Moralli et al. BMC Cell Biology 2009 10:18   doi:10.1186/1471-2121-10-18

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