Figure 5.

Analysis of HAC replication timing. To identify the S phase stage, the cells from clone Sag1.2 were stained with an anti-BrdU antibody (red signals). HAC were identified by FISH with a 17α probe (green signal). DNA was counterstained with DAPI (blue). Examples of early (E), mid (M) and late (L) replicating HAC are shown.