Analysis of HAC replication timing. To identify the S phase stage, the cells from clone Sag1.2 were stained with an anti-BrdU antibody (red signals). HAC were identified by FISH with a 17α probe (green signal). DNA was counterstained with DAPI (blue). Examples of early (E), mid (M) and late (L) replicating HAC are shown.
Moralli et al. BMC Cell Biology 2009 10:18 doi:10.1186/1471-2121-10-18