Subcellular localisation of Cep70 and Cep131 fragments. The N-terminus of Cep70 localises to the centrosome (A) whereas the C-terminus does not (B). Both the N-terminus, IQ (blue square) and HDAC-ID (red hexagon) domains of Cep131 are required for centrosomal localisation (F). (I) depicts which fragments were fused to GFP and the panels in which they are represented. (G) and (H) show aggregates generated by overexpression GFP fusions of the N-terminal fragment and full-length Cep70. GFP fusions are shown in green, gamma tubulin is in red, nuclei are in blue (DAPI) in merged pictures. Scale bar: 10 μm.
Wilkinson et al. BMC Cell Biology 2009 10:17 doi:10.1186/1471-2121-10-17