Image analysis comparison of cell deposited material at cell periphery for cycloheximide treated cells compared to untreatedcells. A) Representative SPR image of vSMC 24 h after plating and exposure to cycloheximide. B) Representative SPR image of untreated vSMC 24 h after plating for comparison. Each image is overlaid with contour outlines depicting the distance-to-cell-edge coverage analysis routine (described in text) shown here at 20 μm from cell edge. C) The image extracted coverage versus distance-to-cell-edge data is displayed for vSMC that are treated and untreated with cycloheximide. The data represent the average of 5 different 500 μm by 500 μm fibronectin patterned regions and the error bars represent the standard deviation.
Peterson et al. BMC Cell Biology 2009 10:16 doi:10.1186/1471-2121-10-16