Additional file 3.

Analysis of the effect of EGFP-DP fusion proteins on cell death of HtTA cells. (A) EGFP-DP fusion proteins induced cell death. HtTA cells were transfected with 0.3 μg of the indicated expression vectors for the EGFP-DP fusion protein for 24 or 48 h. Cell death was detected by measuring LDH release, and cell viability was measured with the MTT method. Representative results of three independent experiments are shown and are expressed as means and standard errors of the means after normalization to the control EGFP group. Student t test: *, P < 0.05; **, P < 0.01. Cells were transfected with expression vectors for the EGFP (B), or various mutated DP fusion proteins (C) for 48 h. Cells were stained with Hoechst 33258 dye, and chromatin condensation was evaluated with a fluorescent microscope. Arrows indicate cells expressing the EGFP-DP fusion protein that were positive for chromatin condensation. Arrowheads indicate mitotic cells. Scale bar: 1 μm.

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Tsai et al. BMC Cell Biology 2009 10:15   doi:10.1186/1471-2121-10-15