Time course of changes in the fluorescence of BSA incubated with different saccharides. BSA (final concentration 150 μM) in the presence of D-ribose (rib, final concentration 1 M) was kept at 37°C in Tris-HCl buffer (pH 7.4). BSA or rib alone was used as a control. (A) Aliquots were taken for measurements of fluorescence spectra (λex = 320 nm) at different time intervals. (B) Time course of changes in the maximal fluorescent intensity (λex320 nm; λem410 nm) of BSA incubated with rib, glucose (glc), fructose (frc), xylose (xyl) and sucrose (suc). (C) Changes in fluorescence at λex 370 nm; λem 425 nm were also detected under the same conditions.
Wei et al. BMC Cell Biology 2009 10:10 doi:10.1186/1471-2121-10-10