Elk-1 coimmunoprecipitates with p35-C/EBPβ, but only in the presence of activated Ras. COS-7 cells were transfected with 10 μg of pCDNA3.1/His-p35-C/EBPβ (lanes 1, 2, 5, 6) and 10 μg of pCMV-Elk-1 (lanes 3, 4, 5, 6) in the presence and absence of 2 μg of pCMV-Ras.V12 as indicated. Cells were harvested 40 h post-transfection, and whole cell lysates were incubated with T7tag Ab-agarose beads, followed by immunoblotting of precipitated proteins with Elk-1 Ab.
Hanlon et al. BMC Cell Biology 2000 1:2 doi:10.1186/1471-2121-1-2