BMC Bioinformatics

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This article is part of the supplement: Asia Pacific Bioinformatics Network (APBioNet) Sixth International Conference on Bioinformatics (InCoB2007)

Open Access Proceedings

In silico analysis of expressed sequence tags from Trichostrongylus vitrinus (Nematoda): comparison of the automated ESTExplorer workflow platform with conventional database searches

Shivashankar H Nagaraj1, Robin B Gasser2, Alasdair J Nisbet3 and Shoba Ranganathan1,4*

Author Affiliations

1 Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW 2109, Australia

2 Department of Veterinary Science, The University of Melbourne, Werribee, VIC 3030, Australia

3 Parasitology Division, Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, EH26 0PZ, UK

4 Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119260

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BMC Bioinformatics 2008, 9(Suppl 1):S10 doi:10.1186/1471-2105-9-S1-S10

Published: 13 February 2008

Additional files

Additional file 1:

Comparison of 696 rESTs from Trichostrongylus vitrinus with Caenorhabditis elegans proteome (Wormpep v 167). The table also provides corresponding RNAi phenotypic information.

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Additional file 2:

Gene Ontology mappings for Trichostrongylus vitrinus rESTs.

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Additional file 3:

Metabolic pathways in Trichostrongylus vitrinus mapped by Kyoto Encyclopedia of Genes and Genomes (KEGG).

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Additional file 4:

An example output for functional classification of ESTs generated using the Blast2GO component of ESTEXplorer's Phase II – Nucleotide-level Analysis.

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Additional file 5:

Comparison of individual EST analysis via BLAST searches and ensemble annotation by ESTExplorer.

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Additional file 6:

Validation of Caenorhabditis elegans smat file used for ESTScan by BLASTX results for untranslated EST sequences.

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