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Open Access Methodology article

Simultaneous fitting of real-time PCR data with efficiency of amplification modeled as Gaussian function of target fluorescence

Anke Batsch1, Andrea Noetel1, Christian Fork1, Anita Urban1, Daliborka Lazic1, Tina Lucas1, Julia Pietsch1, Andreas Lazar1, Edgar Schömig1,2 and Dirk Gründemann1,2*

Author Affiliations

1 Department of Pharmacology, University of Cologne, Gleueler Straße 24, 50931 Cologne, Germany

2 Center for Molecular Medicine (CMMC), University of Cologne, Joseph-Stelzmann-Straße 52, 50931 Cologne, Germany

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BMC Bioinformatics 2008, 9:95 doi:10.1186/1471-2105-9-95

Published: 12 February 2008

Additional files

Additional file 1:

Program SimFitEAv. The main program for PCR data analysis to be used as pro Fit (Quantum Soft, Switzerland) plug-in.

Format: TXT Size: 14KB Download file

Open Data

Additional file 2:

Function EAv. Function called from SimFitEAv to be used as pro Fit (Quantum Soft, Switzerland) plug-in.

Format: TXT Size: 1KB Download file

Open Data

Additional file 3:

Function EAvPeak. Function called from SimFitEAv to be used as pro Fit (Quantum Soft, Switzerland) plug-in.

Format: TXT Size: 1KB Download file

Open Data

Additional file 4:

Function M16EAv. Function called from SimFitEAv to be used as pro Fit (Quantum Soft, Switzerland) plug-in.

Format: TXT Size: 2KB Download file

Open Data

Additional file 5:

Original raw data. Excel file with raw fluorescence and cycle data exported from LightCycler.

Format: XLS Size: 126KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data