Computing Oi Values. This figure provides an overview of Oi generation. Each protein undergoes an in silico trypsin digestion to form peptide sequences that are fed into a classifier trained on previously constructed training data. The classifier generates peptide detection probabilities. Peptide probabilities related to a particular protein i are summed to arrive at the protein's Oi value, or predicted detected spectral count for one instance of the protein.
Braisted et al. BMC Bioinformatics 2008 9:529 doi:10.1186/1471-2105-9-529