Table 3 |
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|
Experimental results of primer pairs designed with QuantPrime |
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|
Experiment |
Predicted gDNA-safe |
Quality control passed1 |
Quality control passed1 for detectable transcripts2 |
|
|
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|
A. thaliana |
113/128 (88.3%) |
117/128 (91.4%) |
117/122 (95.9%) |
|
C. reinhardtii |
24/33 (72.7%) |
28/33 (84.8%) |
28/29 (96.6%) |
|
H. vulgare3 |
- |
27/30 (90.0%) |
27/28 (96.4%) |
|
|
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|
137/161 (85.1%) |
172/191 (90.1%) |
172/179 (96.1%) |
|
|
|
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|
The primer pairs were designed for wet-lab quantification experiments. The number of primer pairs passing strict quality control checks (melting curve analysis, agarose gel separation and efficiency check) are reported in the table. 1 Melting curve analysis, gel analysis and efficiency check (E ≥ 1.8) passed. 2 Undetectable transcripts (Ct > 40) removed from the statistics. 3 TIGR Transcript Assembly annotation used, no genomic sequences available. |
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Arvidsson et al. BMC Bioinformatics 2008 9:465 doi:10.1186/1471-2105-9-465 |
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