BMC Bioinformatics

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Open Access Highly Access Software

QuantPrime – a flexible tool for reliable high-throughput primer design for quantitative PCR

Samuel Arvidsson1,2, Miroslaw Kwasniewski1,2,3, Diego M Riaño-Pachón1,2 and Bernd Mueller-Roeber1,2*

Author Affiliations

1 Max-Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany

2 Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Straße 24-25, Haus 20, 14476 Potsdam-Golm, Germany

3 Department of Genetics, University of Silesia, Jagiellonska 28, 40032, Katowice, Poland

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BMC Bioinformatics 2008, 9:465 doi:10.1186/1471-2105-9-465

Published: 1 November 2008

Additional files

Additional file 1:

List of customizable parameters in QuantPrime. A comprehensive list of all parameters that can be customized in QuantPrime, with parameter ranges and default values.

Format: XLS Size: 16KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 2:

Examples of primer pairs with gel images. Examples of primer pairs for different species with images of agarose gel separations of their PCR amplification products.

Format: PDF Size: 140KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 3:

Comparison of QuantPrime with other primer design software. A comparison table including QuantPrime and other commonly used primer design software.

Format: XLS Size: 28KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data