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Open Access Database

FUNYBASE: a FUNgal phYlogenomic dataBASE

Sylvain Marthey1, Gabriela Aguileta12, François Rodolphe1, Annie Gendrault1, Tatiana Giraud2*, Elisabeth Fournier3, Manuela Lopez-Villavicencio4, Angélique Gautier5, Marc-Henri Lebrun6 and Hélène Chiapello1*

Author Affiliations

1 UR MIG, INRA, Bâtiment 233 Domaine de Vilvert 78350, Cedex, France

2 UMR ESE, Université Paris-Sud, CNRS, AgroParisTech, Bâtiment 360, Université Paris-Sud, 91405 Orsay, Cedex France; CNRS 91405 Orsay, France

3 UMR BGPI, INRA, CIRAD, AgroSup, TA A 54/K, Campus International de Baillarguet, 34398, Montpellier, cedex 5, France

4 MNHN, Département Systématique et Evolution, 12 rue Buffon CP 39, 75005 Paris, France

5 UMR BIOGER, INRA, AgroParisTech, Centre INRA de Versailles, Route de Saint Cyr 78026 ,Versailles, France

6 UMR MAP Université Lyon-1, CNRS, INSA, BAYER CS, 14, rue Pierre Baizet 69009 Lyon, France

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BMC Bioinformatics 2008, 9:456  doi:10.1186/1471-2105-9-456

Published: 27 October 2008

Abstract

Background

The increasing availability of fungal genome sequences provides large numbers of proteins for evolutionary and phylogenetic analyses. However the heterogeneity of data, including the quality of genome annotation and the difficulty of retrieving true orthologs, makes such investigations challenging. The aim of this study was to provide a reliable and integrated resource of orthologous gene families to perform comparative and phylogenetic analyses in fungi.

Description

FUNYBASE is a database dedicated to the analysis of fungal single-copy genes extracted from available fungal genomes sequences, their classification into reliable clusters of orthologs, and the assessment of their informative value for phylogenetic reconstruction based on amino acid sequences. The current release of FUNYBASE contains two types of protein data: (i) a complete set of protein sequences extracted from 30 public fungal genomes and classified into clusters of orthologs using a robust automated procedure, and (ii) a subset of 246 reliable ortholog clusters present as single copy genes in 21 fungal genomes. For each of these 246 ortholog clusters, phylogenetic trees were reconstructed based on their amino acid sequences. To assess the informative value of each ortholog cluster, each was compared to a reference species tree constructed using a concatenation of roughly half of the 246 sequences that are best approximated by the WAG evolutionary model. The orthologs were classified according to a topological score, which measures their ability to recover the same topology as the reference species tree. The full results of these analyses are available on-line with a user-friendly interface that allows for searches to be performed by species name, the ortholog cluster, various keywords, or using the BLAST algorithm. Examples of fruitful utilization of FUNYBASE for investigation of fungal phylogenetics are also presented.

Conclusion

FUNYBASE constitutes a novel and useful resource for two types of analyses: (i) comparative studies can be greatly facilitated by reliable clusters of orthologs across sets of user-defined fungal genomes, and (ii) phylogenetic reconstruction can be improved by identifying genes with the highest informative value at the desired taxonomic level.