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Open AccessMethodology article

ReRep: Computational detection of repetitive sequences in genome survey sequences (GSS)

Thomas D Otto1,2 email, Leonardo HF Gomes1,3 email, Marcelo Alves-Ferreira1 email, Antonio B de Miranda1 email and Wim M Degrave1 email

1Laboratory for Functional Genomics and Bioinformatics, IOC, Fiocruz, Rio de Janeiro, Brazil

2Fundação Ataulpho de Paiva, Rio de Janeiro, Brazil

3Medicine Faculty, UFRJ, Rio de Janeiro, Brazil

author email corresponding author email

BMC Bioinformatics 2008, 9:366doi:10.1186/1471-2105-9-366

Published: 9 September 2008

Additional files

Additional file 1:

Sequences and Sequence Landscapes of the PRS. The DNA sequence and SL of PRS_1 – PRS_5 is given, using l = 400.

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Additional file 2:

PCR reaction for the elements PRS_1. Column MW: 1 kb marker plus DNA Ladder. Size of the bands is indicated on the left. Column 1: amplification with primers: TTGTAAAACGACGGCCAGTG and CACACAGGAAACAGCTATGAC.

Format: PDF Size: 172KB Download file

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Additional file 3:

Multiple alignment for PRS_1. Multiple alignment of read GenBank: EI185111 and read GenBank: EI185194, representing PRS_1.

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Additional file 4:

Multiple alignment of the Tan-PRS_1 elements. The tandem elements of PRS_1 were extracted from the original GSS. Elements A1-A11 were obtained from GenBank: EI185111; elements B1-B10 from GenBank: EI185194.

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Additional file 5:

Table of repeats of E. coli K12. The name, the number of copies and the size of the repeats that are present in the E. coli K12 genome are listed, with indication, which of those are detected by ReRep analysis, with the corresponding parameter values of l and t.

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Additional file 6:

FASTA file of repeats of E. coli K12.

Format: PDF Size: 97KB Download file

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