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Examples of anNET applications |
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| Application |
Ranges estimated |
Notes |
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| Check thermodynamic consistency |
none |
Check only once feasibility. For non-feasible systems, use the troubleshooting routine. |
| Estimate unmeasured concentrations |
concentrations |
|
| Resolve concentrations in different compartments |
concentrations |
|
| Find minimum/maximum feasible NADH/NAD ratio or adenylate energy charge |
Non-linear terms |
Define the ratio of interest as input with very loose (wide) bounds |
| Infer reaction direction |
ΔrG' |
Find reactions that have a ΔrG' with a unique sign |
| Verify reversibility in model |
ΔrG' |
Infer reaction directions from NET analysis and compare with reversibility in model or literature |
| Spot putative control sites |
ΔrG' |
Find reactions that are known to be active and operate far from equilibrium |
| Exclude activity of transporters |
ΔrG' |
Transporters with non-zero, positive ΔrG' are either not active or have reversed flux. |
Zamboni et al. BMC Bioinformatics 2008 9:199 doi:10.1186/1471-2105-9-199 |
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