Figure 1.

Two layouts with the same canvas and three subcellular localizations. The grid canvases (a) and (b) have the same biological subcellular localizations extracellular space, plasma membrane, and cytoplasm. Both canvases contain the same graph with four nodes that are located in plasma membrane, which surrounds cytoplasm. In (a), nodes are spread apart in plasma membrane, and edges among these nodes cross over cytoplasm. In (b), the nodes are gathered in the left-top corner, and no edge crosses over cytoplasm. Due to its crossing patterns in (a) these edges have a higher probability to cross other nodes in cytoplasm. This is the drawback of using the layout in (a) as the initial layout for Eades initial layout algorithm.