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Open Access Highly Accessed Database

PARPs database: A LIMS systems for protein-protein interaction data mining or laboratory information management system

Arnaud Droit12, Joanna M Hunter3, Michèle Rouleau1, Chantal Ethier1, Aude Picard-Cloutier1, David Bourgais1 and Guy G Poirier12*

Author Affiliations

1 Health and Environment Unit, Laval University Medical research Center, CHUQ, Québec, Canada

2 Eastern Quebec Proteomic Center, CHUQ, Quebec, Canada

3 Caprion Pharmaceuticals, Montréal, Canada

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BMC Bioinformatics 2007, 8:483  doi:10.1186/1471-2105-8-483

Published: 19 December 2007

Abstract

Background

In the "post-genome" era, mass spectrometry (MS) has become an important method for the analysis of proteins and the rapid advancement of this technique, in combination with other proteomics methods, results in an increasing amount of proteome data. This data must be archived and analysed using specialized bioinformatics tools.

Description

We herein describe "PARPs database," a data analysis and management pipeline for liquid chromatography tandem mass spectrometry (LC-MS/MS) proteomics. PARPs database is a web-based tool whose features include experiment annotation, protein database searching, protein sequence management, as well as data-mining of the peptides and proteins identified.

Conclusion

Using this pipeline, we have successfully identified several interactions of biological significance between PARP-1 and other proteins, namely RFC-1, 2, 3, 4 and 5.