A procedure for the estimation over time of metabolic fluxes in scenarios where measurements are uncertain and/or insufficient

Francisco Llaneras and Jesús Picó

Dept. of Systems Engineering and Control, Technical University of Valencia, Camino de Vera s/n, 46022 Valencia, Spain

author email corresponding author email

BMC Bioinformatics 2007, 8:421doi:10.1186/1471-2105-8-421


Published:	30 October 2007

Additional files

Additional file 3:

Conversion of measured concentrations to measured fluxes. Example of the calculation of the measured fluxes when the measurements of concentration are obtained at a high sample rate.

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Additional file 2:

Flux spectrum approach in the underdetermined and redundant case. Example of the estimation of fluxes with the Flux Spectrum Approach in an Underdetermined and redundant case.

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Additional file 1:

Metabolic network description. List of metabolites, reactions and stoichiometric matrixes.

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Additional file 4:

Mathematical model (SBML and matlab). The zip file contains the mathematical model of the metabolism of CHO cells in three different formats: a matlab script, an SBML model and a metatool file.

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Additional file 7:

Additional figures and tables. Additional figures cited in the manuscript.

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Additional file 6:

Analysis of the unbalanced propagation of the uncertainty. Dataset with the interval size of the estimations of each non-measured flux at each time instant.

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Additional file 8:

Analysis of the effect of the uncertainty of each measured flux. Details and datasets of the indirect and direct analysis of the effect of the uncertainty of each measured flux.

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Additional file 5:

Implementation of the Flux Spectrum Approach. The zip file contains a matlab script which implements the method to estimate the non-measured fluxes with the Flux Spectrum Approach and a simple example that illustrates how to use it.

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