Resolution:
## Figure 5.
(a and b) Correlation between experimental ln(k. Correlations (a and b) are for differences in ln(k_{cat}/K_{m}) and electrostatic potential differences for TPI and (c and d) prediction of k_{cat}/K_{m }for TPI for the substrate glyceraldehyde-3-phosphate_{cat}/K_{m}) and electrostatic potential differences of each TPI protein pair, after excluding
TPI1_GIALA and TPIS_RABIT. The straight lines correspond to the best linear fit and
are given by y = 1.59*x (a) and y = 0.95*x (b). Predictions (c and d) were made for
10 TPIs based on experimental measurements for the two TPIs (from V. marinus (TPIS_VIBMA) and P. falciparum (TPIS_PLAFA), see Table 2) at the minimum and maximum points on the y = x line, respectively.
These 2 cases were selected to derive the correlation factor α in equation (1). Then
this factor was used to compute the kinetic parameter in the other cases – there are
2 predictions for each case and their deviation defines the error bars. Two outliers
(with errors greater than 1.5 RMS deviation from y = x) are labeled, see text. Correlations
and predictions for (a) and (c) were done using Modeller protein structural models,
whereas SwissModel models were used for the correlations and predictions presented
in (b) and (d).
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