TomoJ: tomography software for three-dimensional reconstruction in transmission electron microscopy
1 Institut Curie. Section Recherche. Laboratoire d'Imagerie Intégrative. Centre Universitaire d'Orsay, 91405 Orsay CEDEX, France
2 INSERM U 759. Centre Universitaire d'Orsay. Bât 112. 91405 Orsay CEDEX, France
3 Bioengineering Lab, Escuela Politécnica Superior. Univ. San Pablo – CEU. Campus Urb. Montepríncipe s/n. 28668. Boadilla del Monte, Madrid, Spain
BMC Bioinformatics 2007, 8:288 doi:10.1186/1471-2105-8-288Published: 6 August 2007
Transmission electron tomography is an increasingly common three-dimensional electron microscopy approach that can provide new insights into the structure of subcellular components. Transmission electron tomography fills the gap between high resolution structural methods (X-ray diffraction or nuclear magnetic resonance) and optical microscopy. We developed new software for transmission electron tomography, TomoJ. TomoJ is a plug-in for the now standard image analysis and processing software for optical microscopy, ImageJ.
TomoJ provides a user-friendly interface for alignment, reconstruction, and combination of multiple tomographic volumes and includes the most recent algorithms for volume reconstructions used in three-dimensional electron microscopy (the algebraic reconstruction technique and simultaneous iterative reconstruction technique) as well as the commonly used approach of weighted back-projection.
The software presented in this work is specifically designed for electron tomography. It has been written in Java as a plug-in for ImageJ and is distributed as freeware.