Effects of scaling on the variance of the six technical replicates of the pooled invertebrate muscle sample. Each plot shows the variance of every bin versus the bin number, where the bin numbers have been ranked according to their mean intensities; i.e. the highest intensity bins appear on the right of each plot. Bin variances are shown for (A) unscaled data, where the insert shows a zoomed in section, (B) autoscaled data, (C) Pareto scaled data, (D) glog transformed data.
Parsons et al. BMC Bioinformatics 2007 8:234 doi:10.1186/1471-2105-8-234