Table 3

BLAST scores of added reactions in S. cerevisiae

Reactions
E Value
Reaction in S. cerevisiae
Organism

methionine γ-lyase
1e-58,1e-68
Cystathionine gamma-lyase
Pseudomonas putida
2-hydroxyglutarate synthase(no gene identified yet)
----------
---------------
E. coli
2-oxoglutarate reductase/phosphoglycerate dehydrogenase
2e-14, 3e-18
3-phosphoglycerate dehydrogenase
Bacillus subtilis
deoxyribose-phosphate aldolase
7.7, 0.006
Phosphatidylinositol 3-kinase TOR2
Mycoplasma pneumoniae
D-lactate dehydrogenase
2, 3e-04
Protein of unknown function
E. coli
L-tryptophan aminotransferase
no information
no information
Enterobacter cloacae
Methylglyoxal synthase
1.5, 0.001
carbamyl phosphate synthetase
E. coli
phenylpyruvate decarboxylase
3E-35, 1e-39
Chain A, Pyruvate Decarboyxlase
Azoarcus sp. EbN1
tagatose-1,6-bisphosphate aldolase 2
1e-12, 1e-16
Fructose 1,6-bisphosphate aldolase
E. coli
tryptophan 2-monooxygenase
2.2, 5e-05
orf:PZA105
E. coli
5,10-methylenetetrahydrofolate reductase(present in the cytosol)
1E-38, 2e-42
Isozyme of methylenetetrahydrofolate reductase
E. coli
adenine phosphoribosyltransferase(present in cytosol)
2E-25, 1e-29
Adenine phosphoribosyltransferase
E. coli
guanylate kinase(present in cytosol)
9E-38, 7e-42
Guanylate kinase
E. coli
putative NAD+ kinase(present in cytosol)
2E-27, 6e-28
ATP-NADH kinase
E. coli
adenylate kinase(present in cytosol and mitochondria)
7E-51, 5e-55
Adenylate kinase
E. coli

Candidate reactions from the MetaCyc database (along with BLAST scores) added to enable production of no-production metabolites in S. cerevisiae.

Satish Kumar et al. BMC Bioinformatics 2007 8:212   doi:10.1186/1471-2105-8-212