Figure 1.

Block diagram of typical clone sequence verification workflow. The diagram illustrates a typical workflow for the full length sequence validation of a protein coding clone. The process, which is described in detail in the text, is an iterative process that collects sequence reads, assembles contigs, finds gaps in coverage, finds regions of low confidence, compares the contig sequence with the expected sequence, and determines the overall acceptability of the clone. Processing steps in parentheses and italics are optional.