A novel approach to sequence validating protein expression clones with automated decision making

Elena Taycher¹ , Andreas Rolfs¹ , Yanhui Hu¹ , Dongmei Zuo¹^,2 , Stephanie E Mohr² , Janice Williamson¹ and Joshua LaBaer¹^,2

¹Harvard Institute of Proteomics, Harvard Medical School, 320 Charles St., Cambridge, MA 02141, USA

²DF/HCC DNA Resource Core, Harvard Medical School, 320 Charles St., Cambridge, MA 02141, USA

author email corresponding author email

BMC Bioinformatics 2007, 8:198doi:10.1186/1471-2105-8-198


Published:	13 June 2007

Additional files

Additional file 1:

Screen-shots of ACE interfaces. Figure `. IsolateRanking Report. Figure 2. Request for Approval of Specific Primers. Figure 3. Decision Tool Execution. Figure 4. Create New Set ofParameters for Clone Ranking. Figure 5. Online example of Gap MapperResult. Figure 6. Online example of Low Confidence Region Finder Results. Figure 7. Parameter Settings for Sequencing Primer Design.

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Additional file 2:

Samples of ACE reports. The file contains sample reports for Decision Tool, Primer Designer, Primer Order, Gap Mapper and Low Confidence Finder.

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Additional file 3:

ACE Data Import and Export. The file contains the organization and type of data that is either imported into or exported from ACE.

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Additional file 4:

XML file with reference sequence descriptions. Example of XML file contains descriptions of reference sequences for all clones on the plate 'YGS000374-1'.

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Additional file 5:

XML file with clone mapping information. Example of XML file contains clone mapping data for the plate 'YGS000374-1'.

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Additional file 6:

Help file. This is the pdf version of the online help files in the software. It includes both a tutorial and an overview of the software, as well as installation instructions.

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