Table 2

Parameters influencing the efficiency of qPCR reactions.

Parameter

Df

F-value

p-value


Amplicon

6

219.331

<0.001

Sample

10

7.227

<0.001

Ct

1

0.646

0.422

Dilution

4

2.111

0.078

Amplicon:Sample

15

4.525

<0.001

Amplicon:Ct

6

1.957

0.07

Amplicon:Dilution

20

2.576

<0.001

Sample:Ct

10

1.226

0.271

Sample:Dilution

37

1.512

0.029

Ct: Dilution

4

1.872

0.114

Residuals

700


A multiple ANOVA test was performed on efficiencies obtained from 700 reactions. Dependence with amplicon sequence, sample, Ct and dilutions were tested as well as all combinations of co-dependence. Df indicates the degree of freedom. The larger the F-value, or the smaller the p value, the more significant is the effect of the corresponding parameter.

Karlen et al. BMC Bioinformatics 2007 8:131   doi:10.1186/1471-2105-8-131

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