Table 1

Comparison between serial dilution and LinReg for the measurement of efficiency

Amplicon

Test of equality*

Test of variance**


Cav

p = 0.24

p < 0.001

CTGF

p < 0.001

p < 0.001

Eln

p = 1

p < 0.001

FN

p = 0.65

p < 0.001

L27

p = 0.8

p < 0.001

Perl

p = 1

p < 0.001

PAI-1

p = 0.93

p < 0.001


* Single one-way ANOVA was applied to the complete set of data to assess whether both the Serial dilution and the LinReg methods give similar averaged efficiencies (H0: efficiencies are equal, H1: efficiencies are different): a p-value below 0.05 indicates that measured efficiencies are significantly different. **F-test assessing whether the variance (error) induced by each method is the same [H0: variances are equal, H1: var(serial dilution) > var(LinReg)]: p < 0.001 indicates that the variance obtained with the serial dilution is significantly greater than that of LinReg.

Karlen et al. BMC Bioinformatics 2007 8:131   doi:10.1186/1471-2105-8-131

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