BMC Bioinformatics

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Statistical significance of quantitative PCR

Yann Karlen, Alan McNair, Sébastien Perseguers, Christian Mazza and Nicolas Mermod*

BMC Bioinformatics 2007, 8:131 doi:10.1186/1471-2105-8-131

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BioMed Central: 11 citations

Research article   Open Access

Receptors and effects of gut hormones in three osteoblastic cell lines

Elda L Pacheco-Pantoja, Lakshminarayan R Ranganath, James A Gallagher, Peter JM Wilson, William D Fraser BMC Physiology 2011, 11:12 (29 July 2011)

The expression of gut hormone receptors, biochemical bone markers and viability in three osteosarcoma cell lines in response to gut hormones varies depending on the stage of osteoblastic development represented by each cell line.

Methodology article   Open Access

Efficiency clustering for low-density microarrays and its application to QPCR

Eric F Lock, Ryan Ziemiecki, JS Marron, Dirk P Dittmer BMC Bioinformatics 2010, 11:386 (20 July 2010)

Methodology article   Open Access Highly Accessed

A rapid and inexpensive labeling method for microarray gene expression analysis

Mario Ouellet, Paul D Adams, Jay D Keasling, Aindrila Mukhopadhyay BMC Biotechnology 2009, 9:97 (25 November 2009)

Research article   Open Access Highly Accessed

Evaluation of the impact of single nucleotide polymorphisms and primer mismatches on quantitative PCR

Brian Boyle, Nancy Dallaire, John MacKay BMC Biotechnology 2009, 9:75 (28 August 2009)

Methodology   Open Access

Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type I

Alina Rodríguez-Mallon, Yamilet Cárdenas, Juana Lugo, Aymé Oliva, Antonio Morales, Mario Estrada Biological Procedures Online 2009, 11:79-98 (10 March 2009)

Research article   Open Access Highly Accessed

A comprehensive collection of experimentally validated primers for Polymerase Chain Reaction quantitation of murine transcript abundance

Athanasia Spandidos, Xiaowei Wang, Huajun Wang, Stefan Dragnev, Tara Thurber, Brian Seed BMC Genomics 2008, 9:633 (24 December 2008)

Methodology article   Open Access Highly Accessed

Critical evaluation of methods used to determine amplification efficiency refutes the exponential character of real-time PCR

Robert G Rutledge, Don Stewart BMC Molecular Biology 2008, 9:96 (30 October 2008)

Measurement of template quantity in real-time qPCR using standard curves is prone to error, and the novel linear regression of efficiency method that calculates the amplification efficiency at the onset of each reaction is more accurate.

Methodology article   Open Access Highly Accessed

A new real-time PCR method to overcome significant quantitative inaccuracy due to slight amplification inhibition

Michele Guescini, Davide Sisti, Marco BL Rocchi, Laura Stocchi, Vilberto Stocchi BMC Bioinformatics 2008, 9:326 (30 July 2008)

Methodology article   Open Access Highly Accessed

A kinetic-based sigmoidal model for the polymerase chain reaction and its application to high-capacity absolute quantitative real-time PCR

Robert G Rutledge, Don Stewart BMC Biotechnology 2008, 8:47 (8 May 2008)

The LRE (linear regression of efficiency) algorithm for estimating PCR efficiency is a kinetic-based sigmoidal approach that, when coupled with optical calibration, provides data comparison without using standard curves, allowing high-capacity absolute PCR quantification.

Methodology article   Open Access Highly Accessed

Highly accurate sigmoidal fitting of real-time PCR data by introducing a parameter for asymmetry

Andrej-Nikolai Spiess, Caroline Feig, Christian Ritz BMC Bioinformatics 2008, 9:221 (29 April 2008)

Research article   Open Access Highly Accessed

Relative quantification of mRNA: comparison of methods currently used for real-time PCR data analysis

Štefan Čikoš, Alexandra Bukovská, Juraj Koppel BMC Molecular Biology 2007, 8:113 (20 December 2007)