Table 2

ΔCt calculation. The table presents the calculation of ΔCt, which is derived from subtracting Ct number of reference gene from that of the target gene. Con stands for concentration.

Sample

Gene

Con

Ct

Sample

Gene

Con

Ct

ΔCt


Control

Target

10

23.1102

Control

Reference

10

19.7415

3.3687

Control

Target

10

22.9003

Control

Reference

10

19.494

3.4063

Control

Target

10

22.8972

Control

Reference

10

19.3906

3.5066

Control

Target

2

26.5801

Control

Reference

2

21.9838

4.5963

Control

Target

2

26.2139

Control

Reference

2

22.4435

3.7704

Control

Target

2

26.0606

Control

Reference

2

22.57

3.4906

Control

Target

0.4

28.1125

Control

Reference

0.4

24.8109

3.3016

Control

Target

0.4

28.1899

Control

Reference

0.4

24.4327

3.7572

Control

Target

0.4

27.5949

Control

Reference

0.4

24.2342

3.3607

Control

Target

0.08

30.2772

Control

Reference

0.08

26.7319

3.5453

Control

Target

0.08

30.4667

Control

Reference

0.08

26.8206

3.6461

Control

Target

0.08

30.7571

Control

Reference

0.08

26.822

3.9351

Treatment

Target

10

21.7813

Treatment

Reference

10

18.4468

3.3345

Treatment

Target

10

21.7564

Treatment

Reference

10

18.8227

2.9337

Treatment

Target

10

21.641

Treatment

Reference

10

18.3061

3.3349

Treatment

Target

2

23.7965

Treatment

Reference

2

21.2568

2.5397

Treatment

Target

2

23.7571

Treatment

Reference

2

21.0956

2.6615

Treatment

Target

2

23.724

Treatment

Reference

2

20.8473

2.8767

Treatment

Target

0.4

26.3794

Treatment

Reference

0.4

23.2322

3.1472

Treatment

Target

0.4

26.2542

Treatment

Reference

0.4

22.9577

3.2965

Treatment

Target

0.4

25.9621

Treatment

Reference

0.4

23.2415

2.7206

Treatment

Target

0.08

28.5479

Treatment

Reference

0.08

25.4817

3.0662

Treatment

Target

0.08

28.3894

Treatment

Reference

0.08

25.608

2.7814

Treatment

Target

0.08

28.3416

Treatment

Reference

0.08

25.5675

2.7741


Yuan et al. BMC Bioinformatics 2006 7:85   doi:10.1186/1471-2105-7-85

Open Data