Statistical analysis of real-time PCR data

doi:10.1186/1471-2105-7-85

BMC Bioinformatics

Volume 7

Viewing options:

Abstract
Full text
PDF (411KB)
Additional files

Associated material:

Related literature:

Articles citing this article
on BioMed Central
on Google Scholar
on ISI Web of Science
on PubMed Central
Other articles by authors
on Google Scholar

Yuan JS
Reed A
Chen F
Stewart CN

on PubMed

Yuan JS
Reed A
Chen F
Stewart CN
Related articles/pages
on Google

on Google Scholar

on PubMed

Tools:

Post to:

Methodology article

Statistical analysis of real-time PCR data

Joshua S Yuan¹^,2 , Ann Reed³ , Feng Chen¹ and C Neal Stewart Jr¹

¹Department of Plant Sciences, University of Tennessee, Knoxville, TN 37996, USA

²University of Tennessee Institute of Agriculture Genomics Hub, University of Tennessee, Knoxville, TN 37996, USA

³Statistical Consulting Center, University of Tennessee, Knoxville, TN 37996, USA

author email corresponding author email

BMC Bioinformatics 2006, 7:85doi:10.1186/1471-2105-7-85


Published:	22 February 2006

Additional files

Additional file 1:

The SAS program implements the data quality control processes.

Format: SAS Size: 1KB Download file

Additional file 2:

The data provides the input data for Program1_QC.sas and Program3_ANCOVA.sas. The data has grouped the Ct values according to the different combination of sample and gene.

Format: TXT Size: 1KB Download file

Additional file 3:

The abbreviated SAS output for all the analyses.

Format: DOC Size: 49KB Download file

This file can be viewed with: Microsoft Word Viewer

Additional file 4:

The SAS program implements the multiple regression model and derives ΔΔCt.

Format: SAS Size: 1KB Download file

Additional file 5:

The input data for Program2_MR.sas, which has contains sample, gene, concentration and Ct number.

Format: TXT Size: 1KB Download file

Additional file 6:

The SAS program implements the ANCOVA model and derives ΔΔCt.

Format: SAS Size: 1KB Download file

Additional file 7:

The SAS program performs both student t test and Wilcoxon two group tests on the ΔCt to derive ΔΔCt.

Format: SAS Size: 1KB Download file

Additional file 8:

The SAS program is a macro that derives confidence interval for Wilcoxon two group tests.

Format: SAS Size: 5KB Download file

Additional file 9:

The input data for Program4_TW.sas. The data contains only sample name and ΔCt.

Format: TXT Size: 1KB Download file

Additional file 10:

The SAS program performs test for equal slope, grouped slope and adjusted ΔΔCt.

Format: SAS Size: 2KB Download file

Additional file 11:

The input data for Program5_LowQualityData.sas.

Format: TXT Size: 1KB Download file