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Open Access Highly Accessed Methodology article

Statistical analysis of real-time PCR data

Joshua S Yuan12, Ann Reed3, Feng Chen1 and C Neal Stewart1*

Author Affiliations

1 Department of Plant Sciences, University of Tennessee, Knoxville, TN 37996, USA

2 University of Tennessee Institute of Agriculture Genomics Hub, University of Tennessee, Knoxville, TN 37996, USA

3 Statistical Consulting Center, University of Tennessee, Knoxville, TN 37996, USA

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BMC Bioinformatics 2006, 7:85  doi:10.1186/1471-2105-7-85

Published: 22 February 2006

Additional files

Additional file 1:

The SAS program implements the data quality control processes.

Format: SAS Size: 1KB Download file

Open Data

Additional file 2:

The data provides the input data for Program1_QC.sas and Program3_ANCOVA.sas. The data has grouped the Ct values according to the different combination of sample and gene.

Format: TXT Size: 1KB Download file

Open Data

Additional file 3:

The abbreviated SAS output for all the analyses.

Format: DOC Size: 49KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 4:

The SAS program implements the multiple regression model and derives ΔΔCt.

Format: SAS Size: 1KB Download file

Open Data

Additional file 5:

The input data for Program2_MR.sas, which has contains sample, gene, concentration and Ct number.

Format: TXT Size: 1KB Download file

Open Data

Additional file 6:

The SAS program implements the ANCOVA model and derives ΔΔCt.

Format: SAS Size: 1KB Download file

Open Data

Additional file 7:

The SAS program performs both student t test and Wilcoxon two group tests on the ΔCt to derive ΔΔCt.

Format: SAS Size: 1KB Download file

Open Data

Additional file 8:

The SAS program is a macro that derives confidence interval for Wilcoxon two group tests.

Format: SAS Size: 5KB Download file

Open Data

Additional file 9:

The input data for Program4_TW.sas. The data contains only sample name and ΔCt.

Format: TXT Size: 1KB Download file

Open Data

Additional file 10:

The SAS program performs test for equal slope, grouped slope and adjusted ΔΔCt.

Format: SAS Size: 2KB Download file

Open Data

Additional file 11:

The input data for Program5_LowQualityData.sas.

Format: TXT Size: 1KB Download file

Open Data