Methodology article
Statistical analysis of real-time PCR data
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* Corresponding author: C Neal Stewart nealstewart@utk.edu
1 Department of Plant Sciences, University of Tennessee, Knoxville, TN 37996, USA
2 University of Tennessee Institute of Agriculture Genomics Hub, University of Tennessee, Knoxville, TN 37996, USA
3 Statistical Consulting Center, University of Tennessee, Knoxville, TN 37996, USA
BMC Bioinformatics 2006, 7:85 doi:10.1186/1471-2105-7-85
Published: 22 February 2006Additional files
Additional file 1:
The SAS program implements the data quality control processes.
Format: SAS Size: 1KB Download file
Additional file 2:
The data provides the input data for Program1_QC.sas and Program3_ANCOVA.sas. The data has grouped the Ct values according to the different combination of sample and gene.
Format: TXT Size: 1KB Download file
Additional file 3:
The abbreviated SAS output for all the analyses.
Format: DOC Size: 49KB Download file
This file can be viewed with: Microsoft Word Viewer
Additional file 4:
The SAS program implements the multiple regression model and derives ΔΔCt.
Format: SAS Size: 1KB Download file
Additional file 5:
The input data for Program2_MR.sas, which has contains sample, gene, concentration and Ct number.
Format: TXT Size: 1KB Download file
Additional file 6:
The SAS program implements the ANCOVA model and derives ΔΔCt.
Format: SAS Size: 1KB Download file
Additional file 7:
The SAS program performs both student t test and Wilcoxon two group tests on the ΔCt to derive ΔΔCt.
Format: SAS Size: 1KB Download file
Additional file 8:
The SAS program is a macro that derives confidence interval for Wilcoxon two group tests.
Format: SAS Size: 5KB Download file
Additional file 9:
The input data for Program4_TW.sas. The data contains only sample name and ΔCt.
Format: TXT Size: 1KB Download file
Additional file 10:
The SAS program performs test for equal slope, grouped slope and adjusted ΔΔCt.
Format: SAS Size: 2KB Download file
Additional file 11:
The input data for Program5_LowQualityData.sas.
Format: TXT Size: 1KB Download file