Table 3

Predicted protein kinase- phosphoprotein pairs supported by experimental evidence

Substrate protein

Predicted protein kinase

Phosphopeptide *

Scansite score **

Estimated probability ***


Yak1p

Tpk1p

RRK

    SS
LVV

0.145, 0.02

0.6, 0.95

Yak1p

Tpk1p

RRA

    S
LNS

0.127

0.75

Sra1p

Tpk1p

RSR

    S
SVM

0.103

0.8

Acc1p

Snf1p

RAV

    S
VSD

0.145

0.6

Pfk2p

Snf1p

KVH

    S
YTD

0.145

0.6

Hog1p

Ste7p

PQM

    T
G
    Y
VST

0.19

0.33

Bni5p

Cdc28p

PVS

    S
PIT

0.145

0.6

Gpd1p

Bck1p

RSS

    S
SV
    S
LKA

0.141

0.6

Hsp26p

Ume5p

LAN

    T
PAK

0.082

0.85

Msn2p

Pkc1p

RRP

    S
YRR

0.147

0.6

Sok2p

Yak1p

KSI

    S
PRT

0.148

0.6

Ssd1p

Hog1p

SLS

    S
PTK

0.039

0.95

Ste2p

Fus3p

QLP

    T
P
    TS
SKN

0.04

0.99


* The phosphorylated residues are underlined, and the residues not present in the phosphopeptide sequences [4] are shown in italic. When there is more than one phosphorylation site, the one discussed is shown in bold, unless the same protein kinase is predicted for all sites in the peptide.

** Scansite [11] scores were calculated as described in the Methods section. When the same protein kinase is predicted for all sites in the peptide, the scores are given for the respective sites, starting at the N-terminus. If more than one protein kinase yields a similar score, all the possible kinases are listed.

*** Probabilities were calculated as described in the Methods section (Figure 1). When the same protein kinase is predicted for all sites in the peptide, the values are given for the respective sites, starting at the N-terminus.

Brinkworth et al. BMC Bioinformatics 2006 7:47   doi:10.1186/1471-2105-7-47

Open Data