Mapping of PETs to the genome. The 5' and 3' tags of a PET were split and separately mapped to the human genome sequences. Due to the short length of tags and the complexity of the human genome, some of the tags could be mapped non-specifically to multiple locations in the genome. The 5' and 3' tags derived from the same PET were mated based on the criteria that the paired 5' and 3' tags had to be in the correct orientation and order (5'→3'), on the same chromosome, and within the defined appropriate distance.
Chiu et al. BMC Bioinformatics 2006 7:390 doi:10.1186/1471-2105-7-390