Domain interactions. We assume that binding domain one controls the other two domains like indicated by the arrows. From this assumption the kinetic parameters for the model follow immediately. As soon as binding domain one is occupied, the affinities of the docking sites two and three will change. Since binding domain one is independent of the other binding sites, the on- and off-rate constants of this domain are also independent.
Conzelmann et al. BMC Bioinformatics 2006 7:34 doi:10.1186/1471-2105-7-34