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Resolution: standard / high Figure 6.
Screen shot of a sequence read obtained with MAGIC-SPP. The sequence read was obtained from a publicly accessible implementation of MAGIC
DB and is displayed with the MAGIC SeqView GUI available at that web site [22]. The
upper window displays base calls; the lower provides phred quality scores in a histogram,
with the first base call at the left. The quality-trimmed region provided by default
parameters is in orange. The center of the gap at nucleotide 588 is highlighted in
blue in the upper window and marked by a vertical blue line in the lower window. In
a window of 10, the average error probability of this gap is 0.402, sufficient for
Lucy to divide the high-quality region into two segments when used with default parameters
[2]. Lucy would then pick the longest segment as its final output. In contrast, the
length of the right-hand high quality segment is sufficient for the Gap-Join/Seq-Merge
algorithm when used with default parameters to merge the two regions, providing an
additional 102 high quality base calls in the final quality-trimmed output.
Liang et al. BMC Bioinformatics 2006 7:115 doi:10.1186/1471-2105-7-115 |