Figure 9.

Result of assessment of CLPM with a crosslinked sample. (A) BS³ was chosen as the crosslinker to crosslink peptide P, whose sequence is acetyl-AYAGKAGAR (in 95% purity). In the MS spectrum, precursor peaks of both crosslinked and uncrosslinked peptides were seen with different charges. (B) CLPM's output shows that an unacetylated peptide was found to match peak 432.7 (2H+) (light blue) and the crosslinked specie of the acetylated peptide was found to match to peak 650.63 (3H+) (dark blue). The output includes the precursor peak, the peptide sequence, the mass of the peptide, the mass-difference between actual and theoretical masses, the modifications (if any), and the possible modified locations.