Figure 5.

Mechanism of protein in silico digestion. To digest a protein (A) with four cleavable sites C1 to C4 with a missed-cleavage level of 2, CLPM will generate peptides (B) with zero, one and two consecutively missed cleavage sites (shown in green). B located at the left side of the protein represents the N-terminus of the protein and Z represents the C-terminus.