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Open Access Highly Accessed Methodology article

A standard curve based method for relative real time PCR data processing

Alexey Larionov1*, Andreas Krause2 and William Miller3

Author Affiliations

1 Breast Unit, Western general Hospital, Edinburgh, UK

2 Novartis Pharmaceuticals, Biostatistics, CH – 4002 Basel, Switzerland

3 Breast Unit, Edinburgh University, Edinburgh, UK

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BMC Bioinformatics 2005, 6:62  doi:10.1186/1471-2105-6-62

Published: 21 March 2005

Additional files

Additional File 1:

Pdf file with formulae.

Format: PDF Size: 34KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional File 2:

ZIP file containing VBA macros (PCR1.xls), test data for the above macros (Target1.csv, Target2.csv, Target3.csv, Target4.csv, Target5.csv, Reference1.csv, Reference2.csv) and instruction to the above macros (Instructions.pdf). Unzip file into a separate folder on your PC and follow the instructions.

Format: ZIP Size: 313KB Download file

Open Data