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Open Access Database

DSD – An integrated, web-accessible database of Dehydrogenase Enzyme Stereospecificities

Christopher P Toseland, Helen M McSparron and Darren R Flower*

Author Affiliations

The Jenner Institute, University of Oxford, Compton, Berkshire, RG20 7NN, UK

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BMC Bioinformatics 2005, 6:283  doi:10.1186/1471-2105-6-283

Published: 30 November 2005

Abstract

Background

Dehydrogenase enzymes belong to the oxidoreductase class and utilise the coenzymes NAD and NADP. Stereo-selectivity is focused on the C4 hydrogen atoms of the nicotinamide ring of NAD(P). Depending upon which hydrogen is transferred at the C4 location, the enzyme is designated as A or B stereospecific.

Description

The Dehydrogenase Stereospecificity Database v1.0 (DSD) provides a compilation of enzyme stereochemical data, as sourced from the primary literature, in the form of a web-accessible database. There are two search engines, a menu driven search and a BLAST search. The entries are also linked to several external databases, including the NCBI and the Protein Data Bank, providing wide background information. The database is freely available online at: http://www.jenner.ac.uk/DSD/ webcite

Conclusion

DSD is a unique compilation available on-line for the first time which provides a key resource for the comparative analysis of reductase hydrogen transfer stereospecificity. As databases increasingly form the backbone of science, largely complete databases such as DSD, are a vital addition.