Control spot fluorescence and slide print order We expect control spot intensity to be constant across slide print order. The trend, estimated with loess regression, indicates a processing effect due to printing order. In this case, the decrease in control spot intensity, coupled with knowledge of the printing process, suggests that the quantity of printed material is decreasing over a printing run.
Daly et al. BMC Bioinformatics 2005 6:17 doi:10.1186/1471-2105-6-17