Detection of nuclei in 4D Nomarski DIC microscope images of early Caenorhabditis elegans embryos using local image entropy and object tracking

doi:10.1186/1471-2105-6-125

BMC Bioinformatics

Volume 6

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Methodology article

Detection of nuclei in 4D Nomarski DIC microscope images of early Caenorhabditis elegans embryos using local image entropy and object tracking

Shugo Hamahashi¹^,2^,3 , Shuichi Onami¹^,2^,3 and Hiroaki Kitano¹^,2^,4

¹Kitano Symbiotic Systems Project, ERATO, Japan Science and Technology Corporation, M31 6A, 6-31-15 Jingumae, Shibuya, Tokyo 150-0001, Japan

²Graduate School of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku, Yokohama 223-8522, Japan

³Institute for Bioinformatics Research and Development (BIRD), Japan Science and Technology Agency, 5-3 Yonbancho, Chiyoda, Tokyo 102-0081, Japan

⁴Sony Computer Science Laboratories, Inc., 3-14-13 Higashi-Gotanda, Shinagawa, Tokyo 141-0022, Japan

author email corresponding author email

BMC Bioinformatics 2005, 6:125doi:10.1186/1471-2105-6-125


Published:	24 May 2005

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Additional File 1:

Detailed discussions about various measures of image texture.

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Additional File 2:

Figure 5 Low-entropy regions in a par-1 embryo. Input images of a par-1 embryo are shown in the left column and low-entropy regions (black) are shown in the right column. Bar is 10 μm.

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Additional File 3:

Figure 6 Low-entropy regions in a tba-2 (RNAi) embryo. Input images are shown in the left column and low-entropy regions (black) are shown in the right column. Bar is 10 μm.

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