Table 1

Features measured by the automated system. List of the behavioral and morphological features. Detail algorithms to output these features are found in supplemental data. 144 statistical results (mean, maximum and minimum where applicable) of these features are output into Microsoft Access, while the values of these feature at each time when an image is grabbed are saved in Microsoft Excel.

Features

Comments

Catalog


Area

Total area of worm

Body morphology

BendingFrequency

Frequency of body bends

Wave form/speed

CmptFactor

Compactness factor

Body posture

ElgFactor

Elongation factor

Body posture

EllMAxis

Best-fit ellipse, major axis

Body posture

EllRatio

Best-fit ellipse, major axis/minor axis

Body posture

EqvlEllRatio

Equivalent ellipse ratio

Body posture

Fatness

Area/Length

Body morphology

Flex

Maximum skeleton point angle difference

Wave form

Foraging

Frequency of sideways (foraging) head movements

Specific behavior (feeding)

Foraging angle

Angle of foraging movements

Specific behavior (feeding)

Foraging distance

Distance moved by head during foraging

Specific behavior (feeding)

FRE

Frequency of angle change between skeleton points

Wave form/speed

GlbMvScope

Maximum distance moved from starting point

Global movement

GlbSpd

Speed of the animal's centroid movement

Global movement

HdTlRatio

Ratio of head to tail movement speed

Head movement

Heywood

Heywood circularity factor

Body morphology

Hydraulic

Hydraulic radius

Body morphology

IXX

Inertia XX

Body morphology

IXY

Inertia YY

Body morphology

IYY

Inertia XY

Body morphology

LclSpd

Local movement speed

Local speed

Length

Distance from head to tail

Body morphology/posture

LengthToPixel

Length/number of pixels in skeleton

Body posture

Loop

Percentage of time worms coils their body

Specific behavior (coil)

MaxIntercept

Max intercept

Body morphology

MeanIntcptPpdcl

Mean perpendicular intercept

Body morphology

MostPopularArea

Mode of area

Body morphology

MostPopularSpeed

Mode of speed

Global speed

PercentageMPArea

Frequency of occurrence of modal area

Body morphology

PercentageMPSpeed

Frequency of occurrence of modal speed

Global speed

Pushing

Local body movement speed/centroid speed

Locomotion wave efficiency

RectBigSide

Minimum enclosing rectangle (MER) length

Body posture

RectRatio

Minimum enclosing rectangle length/width ratio

Body posture

Reversal

Percentage of time that a worm performs reversals

Specific behavior (escape)

ReversalCount

Number of reversal sessions that a worm performs

Specific behavior (escape)

ReversalDisAve

Average distance traveled backward in reversals

Specific behavior (escape)

SktAglAve

Mean value of skeleton point vector angle

Body bending

SktCmptFactor

Length of worm / oriented MER area

Body posture

SktElgFactor

Skeleton enlongation factor

Body posture

SktHight

Oriented MER height

Body posture

SktIXX

Sum of x coordinate times x coordinates of skeleton points

Body posture

SktIXY

Sum of x coordinate times y coordinates of skeleton points

Body posture

SktIYY

Sum of y coordinate times y coordinates of skeleton points

Body posture

SktvAglAve

Average angle between skeleton points and centroid

Body bending

SktvAglMax

Max angle between skeleton points and centroid

Body bending

SktvDisAveToLength

Average distance between skeleton points and centroid/length

Body bending

SktvDisMaxToLength

Max distance between skeleton points and centroid/length

Body bending

SktvDisMinToLength

Min distance between skeleton points and centroid/length

Body bending

SktWidth

Oriented MER width

Body posture

Theta

Direction of centroid movement

Global movement

Thickness

Thickness of worm

Body morphology

TotalTravelDistance

Total distance traveled by a worm

Global movement

TrackAmplitude

Amplitude of waves in worm's track

Wave form

TrackWavlength

Wavelength of waves in worm's track

Wave form

Transparency

Transparency of worm body

Body morphology

Turn

Percentage of time that a worm performs a sharp turn

Specific behavior (search)

TypeFactor

Type Factor

Body morphology

Waddel

Waddel disk diameter

Body morphology

WaveLenth

Largest spatial span of a sine wave

Wave form

XSym

Sum of X coordinates of all sktps

Body bending

XYSym

Sum f Y coordinates of all sktps

Body bending

YSym

Sum of X coordinate * absolute value of Y coordinate

Body bending


Feng et al. BMC Bioinformatics 2004 5:115   doi:10.1186/1471-2105-5-115

Open Data