Methodology article

Probabilistic estimation of microarray data reliability and underlying gene expression

Sven Bilke¹ , Thomas Breslin¹ and Mikael Sigvardsson²

¹  Complex Systems Division, Department of Theoretical Physics, University of Lund, Sölvegatan 14A, SE-223 62 Lund, Sweden

²  The Laboratory for Cell Differentiation Studies, Department for Stem Cell Biology, BMC B12, SE-22185 Lund, Sweden

author email corresponding author email

BMC Bioinformatics 2003, 4:40doi:10.1186/1471-2105-4-40

Published:	10 September 2003

Abstract

Background

The availability of high throughput methods for measurement of mRNA concentrations makes the reliability of conclusions drawn from the data and global quality control of samples and hybridization important issues. We address these issues by an information theoretic approach, applied to discretized expression values in replicated gene expression data.

Results

Our approach yields a quantitative measure of two important parameter classes: First, the probability P(σ|S) that a gene is in the biological state σ in a certain variety, given its observed expression S in the samples of that variety. Second, sample specific error probabilities which serve as consistency indicators of the measured samples of each variety. The method and its limitations are tested on gene expression data for developing murine B-cells and a t-test is used as reference. On a set of known genes it performs better than the t-test despite the crude discretization into only two expression levels. The consistency indicators, i.e. the error probabilities, correlate well with variations in the biological material and thus prove efficient.

Conclusions

The proposed method is effective in determining differential gene expression and sample reliability in replicated microarray data. Already at two discrete expression levels in each sample, it gives a good explanation of the data and is comparable to standard techniques.