BorreliaBase: a phylogeny-centered browser of Borrelia genomes
1 Department of Biological Sciences, Hunter College, The City University of New York, 10065 New York, NY, USA
2 Department of Computer Science, Hunter College, The City University of New York, 10065 New York, NY, USA
3 Department of Biology, The Graduate Center, City University of New York, 10016 New York, USA
4 Center for Translational and Basic Research, Hunter College, The City University of New York, 10065 New York, NY, USA
5 Institute for Genome Sciences, University of Maryland School of Medicine, 21201 Baltimore, MD, USA
6 Department of Medicine, New Jersey Medical School, Rutgers, The State University of New Jersey, 07103 Newark, NJ, USA
7 Department of Medicine, Health Science Center, Stony Brook University, 11794 Stony Brook, NY, USA
8 Department of Pathology, Division of Molecular Cell Biology and Immunology, University of Utah School of Medicine, 84112 Salt Lake City, UT, USA
9 Department of Biological Sciences, Hunter College of the City University of New York, 695 Park Avenue, 10065 New York, NY, USA
BMC Bioinformatics 2014, 15:233 doi:10.1186/1471-2105-15-233Published: 3 July 2014
The bacterial genus Borrelia (phylum Spirochaetes) consists of two groups of pathogens represented respectively by B. burgdorferi, the agent of Lyme borreliosis, and B. hermsii, the agent of tick-borne relapsing fever. The number of publicly available Borrelia genomic sequences is growing rapidly with the discovery and sequencing of Borrelia strains worldwide. There is however a lack of dedicated online databases to facilitate comparative analyses of Borrelia genomes.
We have developed BorreliaBase, an online database for comparative browsing of Borrelia genomes. The database is currently populated with sequences from 35 genomes of eight Lyme-borreliosis (LB) group Borrelia species and 7 Relapsing-fever (RF) group Borrelia species. Distinct from genome repositories and aggregator databases, BorreliaBase serves manually curated comparative-genomic data including genome-based phylogeny, genome synteny, and sequence alignments of orthologous genes and intergenic spacers.
With a genome phylogeny at its center, BorreliaBase allows online identification of hypervariable lipoprotein genes, potential regulatory elements, and recombination footprints by providing evolution-based expectations of sequence variability at each genomic locus. The phylo-centric design of BorreliaBase (http://borreliabase.org webcite) is a novel model for interactive browsing and comparative analysis of bacterial genomes online.