Splicing event characterization in Drosophila heads. (A) Pairwise splicing events defined for all transcript models in Flybase 5.39 annotation (black bars), and the subset of those detected as alternatively spliced in female and male Drosophila heads by Spanki (grey bars). Black bars indicate splicing event classes as a percent of all defined pairwise events. Grey bars indicate splicing events detected as a percent of all detected events. Cartoons of each event type are in the leftmost column, with the “inclusion” form indicated in green, and the “exclusion” form indicated in orange. A description of each type is adjacent to each cartoon. The “Unclassified” types includes diverse complex type with no concise verbal description. (B) Ratio vs average abundance scatterplots of gene expression in female and male heads. Genes with significant sex-biased gene-level expression (FDR adjusted p < 0.05, Cuffdiff) in females (red), or males (blue). Remaining detected expression events are in grey. Transcripts showing sex-biased splicing have been projected onto the gene-level expression data (green). (C) Scatter plot of percent spliced in (PSI) by RNA-Seq vs qPCR for the inclusion or exclusion form for each event assayed (the PCR primer pairs differ for detecting exon inclusion or exclusion but approximates PSI). Inset is Pearson’s r coefficient.
Sturgill et al. BMC Bioinformatics 2013 14:320 doi:10.1186/1471-2105-14-320