Major steps in building digital neuronal databases and brain atlases. After necessary image processing, the 3D morphology of a neuronal pattern is reconstructed from microscopy data. Multiple neuronal patterns need to be organized based on brain regions and neuronal types. For brain atlases, these reconstructions are mapped into a common 3D spatial coordinate system. This may involve an iterative analysis process.
Peng et al. BMC Bioinformatics 2013 14:293 doi:10.1186/1471-2105-14-293